Difference between revisions of "Part:BBa K4830014:Design"

(Design Notes)
 
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===Design Notes===
 
===Design Notes===
Codon optimization to avoid illegal Restriction sites.
+
Codon optimization
  
 
===Source===
 
===Source===

Latest revision as of 04:46, 12 October 2023


nickase Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization

Source

pCMV-PE2 (H840A) Addgene Plasmid #132775

References

Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.