Difference between revisions of "Part:BBa K239005"

Revision as of 15:41, 20 October 2009

NarK promoter, Fnr activated under anaerobic conditions

Sequence contains 2 Fnr, 1 Fis, 4 NarL and 1 IHF binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions.

Area of application: Detection of when the cell is switching to anaerobic metabolism.

Experiments by Kolesnikow et al. (1992) has showed that NarK expression is upregulated 100-fold under anaerobic condtitions and a further 8-fold in the presence of nitrate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K239005 short</partinfo>
-Sequence contains 2 Fnr, 1 Fis, 4 NarL and 1 IHF binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions (upregulated 100-fold and a further 8-fold in the presence of nitrate). Area of application: Detection of when the cell is switching to anaerobic metabolism.
+Sequence contains 2 Fnr, 1 Fis, 4 NarL and 1 IHF binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions.
+Area of application: Detection of when the cell is switching to anaerobic metabolism.
+Experiments by Kolesnikow et al. (1992) has showed that NarK expression is upregulated 100-fold under anaerobic condtitions and a further 8-fold in the presence of nitrate.
 <!-- Add more about the biology of this part here