Difference between revisions of "Part:BBa K4830034:Design"
Aruzhan001 (Talk | contribs) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | NCBI ACCESSION: AAB59368 | |
| − | + | ||
===References=== | ===References=== | ||
| + | Santos, F.R. (2000) ‘A polymorphic L1 retroposon insertion in the centromere of the human Y chromosome’, Human Molecular Genetics, 9(3), pp. 421–430. doi:10.1093/hmg/9.3.421. | ||
Latest revision as of 03:54, 12 October 2023
Line 1 RT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.
Source
NCBI ACCESSION: AAB59368
References
Santos, F.R. (2000) ‘A polymorphic L1 retroposon insertion in the centromere of the human Y chromosome’, Human Molecular Genetics, 9(3), pp. 421–430. doi:10.1093/hmg/9.3.421.