Difference between revisions of "Part:BBa K4683008"

Latest revision as of 03:43, 12 October 2023

hsa-miR-20b Padlock Probe

This part is the sequence for the hsa-miR-20b Padlock Probe, hsa-miR-20b (BBa_K4683005)is used as the basis for the 3' arm (BBa_K4683006) and 5' arm (BBa_K4683007), When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4683008 short</partinfo>
-This part is the sequence for the hsa-miR-20b Padlock Probe, hsa-miR-20b is used as the basis forthe 3' arm for hsa-miR-20b and 5' arm for hsa-miR-20b,
+This part is the sequence for the hsa-miR-20b Padlock Probe, hsa-miR-20b (<partinfo>BBa_K4683005 </partinfo>)is used as the basis for the 3' arm (<partinfo>BBa_K4683006 </partinfo>) and 5' arm (<partinfo>BBa_K4683007 </partinfo>),
-When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.
+When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from <partinfo>BBa_K4245131 </partinfo> (Middle Sequence), which is then detected by the linear probes <partinfo>BBa_K4245130 </partinfo> (Fluorophore) and <partinfo>BBa_K4245132 </partinfo> (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.