Difference between revisions of "Part:BBa K4759050"

Latest revision as of 03:21, 12 October 2023

GFP11-linker-OleP

Generally, the method of determining whether the redox partners was suitable required tedious steps such as the construction of plasmids, heterologous expression, construction of catalytic systems, and detection of conversion rate after catalysis. Therefore, we wanted to find a convenient way to do a quick screening. We used the fluorescent protein sfGFP and successfully constructed a sensor to detect redox partners. We divided sfGFP into N-terminal and C-terminal, and although these two parts were cut off, there was an interaction force between them. iron redox proteins were fused to the N-terminal of sfGFP-1-10 and Olep to the C-terminal of sfGFP-11, respectively

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 112
Illegal AgeI site found at 210
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4759050 short</partinfo>
-The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities.
+Generally, the method of determining whether the redox partners was suitable required tedious steps such as the construction of plasmids, heterologous expression, construction of catalytic systems, and detection of conversion rate after catalysis. Therefore, we wanted to find a convenient way to do a quick screening. We used the fluorescent protein sfGFP and successfully constructed a sensor to detect redox partners.
+We divided sfGFP into N-terminal and C-terminal, and although these two parts were cut off, there was an interaction force between them. iron redox proteins were fused to the N-terminal of sfGFP-1-10 and Olep to the C-terminal of sfGFP-11, respectively
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