Difference between revisions of "Part:BBa K4949004"
Line 19: Line 19:
  
  
−
<html><img src="https://static.igem.wiki/teams/4949/wiki/results/figure-18-est119-wholecell-lpp-delta-strain.png" width="950" height="700"></html>
+
<html><img src="https://static.igem.wiki/teams/4949/wiki/results/figure-18-est119-wholecell-lpp-delta-strain.png" width="950" height="500"></html>
 
'''Figure 1.''' Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression, <i>E.coli</i> BL21 (DE3) + Est 119 or <i>E.coli</i> BL21 (DE3)ΔLpp + Est119,  a native Lpp deficient stain, were washed and resuspended in PBS with 100µM NPO with a cell density normalized to OD<sub>600</sub>=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.  
 
'''Figure 1.''' Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression, <i>E.coli</i> BL21 (DE3) + Est 119 or <i>E.coli</i> BL21 (DE3)ΔLpp + Est119,  a native Lpp deficient stain, were washed and resuspended in PBS with 100µM NPO with a cell density normalized to OD<sub>600</sub>=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.  
 
<html><br></html>
 
<html><br></html>
  
−
<html><img src="https://static.igem.wiki/teams/4949/wiki/results/figure19-est119-pnob-deltalpp-strain.png" width="500" height="500"></html>
+
<html><img src="https://static.igem.wiki/teams/4949/wiki/results/figure19-est119-pnob-deltalpp-strain.png" width="950" height="500"></html>
 
'''Figure 2.''' Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression, <i>E.coli</i> BL21 (DE3) + Est119 or <i>E.coli</i> BL21 (DE3)ΔLpp +Est119, a native Lpp deficient stain, were washed and resuspended in PBS with 100µM pNOB with a cell density normalized to OD<sub>600</sub>=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.  
 
'''Figure 2.''' Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression, <i>E.coli</i> BL21 (DE3) + Est119 or <i>E.coli</i> BL21 (DE3)ΔLpp +Est119, a native Lpp deficient stain, were washed and resuspended in PBS with 100µM pNOB with a cell density normalized to OD<sub>600</sub>=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.  
 
<html><br></html>
 
<html><br></html>

Revision as of 03:03, 12 October 2023

Status: 500 Content-type: text/html

Software error:

Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


Est119 is an esterase originally identified in the Thermobifida alba strain AHK119 (AB298783). Est119 has been shown to degrade aliphatic-aromatic copolyesters and decrease the size of polymer particles of other biodegradable plastics, with an optimal temperature range of 45-55°C (Hu and al. 2009). Similarly to MGS0156, Est119 is interesting due to its potential temperature compatibility with Manitoban composting methods. Our team modified the Est119 genetic sequence to include the Lpp-OmpA anchor to allow for the characterization of the surface-display mechanism of PLAnet Zero

Sequence and Features Status: 500 Content-type: text/html

Software error:

Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.




Figure 1. Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression, E.coli BL21 (DE3) + Est 119 or E.coli BL21 (DE3)ΔLpp + Est119, a native Lpp deficient stain, were washed and resuspended in PBS with 100µM NPO with a cell density normalized to OD600=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.

Figure 2. Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression, E.coli BL21 (DE3) + Est119 or E.coli BL21 (DE3)ΔLpp +Est119, a native Lpp deficient stain, were washed and resuspended in PBS with 100µM pNOB with a cell density normalized to OD600=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.

Figure 3. Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression E.coli BL21 (DE3) + Est119 or E.coli BL21 (DE3) + Est119(ΔLpp), bacteria with a Lpp deficient plasmid, were washed, and resuspended in PBS with 100µM pNOB with a cell density normalized to OD600=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.

Figure 4. Absorbance as a function of time for the whole cell esterase activity assay of Est119. After induced expression, E.coli BL21 (DE3) + Est119 or E.coli BL21 (DE3) + Est119(ΔLpp), bacteria with an Lpp deficient plasmid, were washed and resuspended in PBS with 100µM NPO with a cell density normalized to OD600=1.0. Samples were then incubated for 5 min at room temperature. Aliquots of 100 µl were collected at 5 intervals and the absorbance of the supernatant as measured at 410 nm.