Difference between revisions of "Part:BBa K200025"

Latest revision as of 14:38, 20 October 2009

pCstA+RBS+RcsB+TT

This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.

The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression of RcsB.

Usage and Biology

This composite part, comprising of subparts BBa_K200020 and BBa_K200024, was used in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project by Imperial iGEM 2009.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 21:33, 18 October 2009 (view source) Royah (Talk \| contribs) ← Older edit		Latest revision as of 14:38, 20 October 2009 (view source) Royah (Talk \| contribs)
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	This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.		This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.
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		+	The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression of RcsB.