Difference between revisions of "Part:BBa K4939001"

Latest revision as of 02:31, 12 October 2023

bmoR

Encodes the BmoR transcription factor, an alcohol responsive bacterial enhancer protein (bEBP) that activates the butanol sensitive pBmo promoter and thus allows for butanol biosensing.

Description

Originally isolated from sludge at a Japanese oil refinery, Thauera butanivorans is a gram negative alkanotroph that can metabolise C2-C9 alkanes. The metabolic operon with which the cells can carry out this metabolism is under the control of pBmo (see BBa_K4939002) - a butanol sensitive, σ54 dependent promoter that itself is regulated by the BmoR repressor (an alcohol-responsive bacterial enhancer protein (bEBP)). In typical σ54 fashion, the activation of the associated operon involves an upstream activation sequence (UAS) some 193 bp upstream of the RNAP binding site - to which a butanol dependant oligomerized BmoR protein binds and enacts transcriptional activation. Therefore, BmoR binding triggers significant DNA bending to achieve gene activation. Thus, for the use of a functional butanol biosensor in vivo, the strain in question must also contain the pBmo and associated UAS. Butanol biosensing has been achieved in E. coli using this system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Encodes the BmoR transcription factor, an alcohol responsive bacterial enhancer protein (bEBP) that activates the butanol sensitive pBmo promoter and thus allows for butanol biosensing.
-Originally isolated from sludge at a Japanese oil refinery, Thauera butanivorans is a gram negative alkanotroph that can metabolise C2-C9 alkanes. The metabolic operon with which the cells can carry out this metabolism is under the control of pBmo - a butanol sensitive, &#963;54 dependent promoter that itself is regulated by the BmoR repressor (an alcohol-responsive bacterial enhancer protein (bEBP)). In typical &#963;54 fashion, the activation of the associated operon involves an upstream activation sequence (UAS) some 193 bp upstream of the RNAP binding site - to which a butanol dependant oligomerized BmoR protein binds and enacts transcriptional activation. Therefore, BmoR binding triggers significant DNA bending to achieve gene activation. Thus, for the use of a functional butanol biosensor in vivo, the strain in question must also contain the pBmo and associated UAS. Butanol biosensing has been achieved in E. coli using this system.
+<partinfo>BBa_K4939000 parameters</partinfo>
+<!-- -->
+===Description===
+Originally isolated from sludge at a Japanese oil refinery, Thauera butanivorans is a gram negative alkanotroph that can metabolise C2-C9 alkanes. The metabolic operon with which the cells can carry out this metabolism is under the control of pBmo (see BBa_K4939002)  - a butanol sensitive, &#963;54 dependent promoter that itself is regulated by the BmoR repressor (an alcohol-responsive bacterial enhancer protein (bEBP)). In typical &#963;54 fashion, the activation of the associated operon involves an upstream activation sequence (UAS) some 193 bp upstream of the RNAP binding site - to which a butanol dependant oligomerized BmoR protein binds and enacts transcriptional activation. Therefore, BmoR binding triggers significant DNA bending to achieve gene activation. Thus, for the use of a functional butanol biosensor in vivo, the strain in question must also contain the pBmo and associated UAS. Butanol biosensing has been achieved in E. coli using this system.