Difference between revisions of "Part:BBa K4645005"
Ruixuan Zhu (Talk | contribs) |
Ruixuan Zhu (Talk | contribs) |
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We assembled P<sub>qtip</sub> with <bbpart>BBa_K4645004</bbpart>then transfected into <i>E. coli</i> to sensing the density of bacteria and regulate the circuit.</P> | We assembled P<sub>qtip</sub> with <bbpart>BBa_K4645004</bbpart>then transfected into <i>E. coli</i> to sensing the density of bacteria and regulate the circuit.</P> | ||
===Characterization=== | ===Characterization=== | ||
| − | <p>We inserted cCFP reporter gene behind qitp promoter to verify whether qtip promoter could work as expected in BL21. However, bacteria that have transformed this plasmid never shown any fluoresce, even though the sequence didn’t mutate. Later, we found that the sequence of qtip promoter contains more than one initiation codon. This may lead to the losing of efficiency of ECFP. So, we tried to delete sequence between eCFP and the last initiation codon and between eCFP and the penult one. Sad to say, we were not able to make this part work finally. | + | <p>We inserted cCFP reporter gene behind qitp promoter to verify whether qtip promoter could work as expected in <i>E. coli</i> BL21(DE3). However, bacteria that have transformed this plasmid never shown any fluoresce, even though the sequence didn’t mutate. Later, we found that the sequence of qtip promoter contains more than one initiation codon. This may lead to the losing of efficiency of ECFP. So, we tried to delete sequence between eCFP and the last initiation codon and between eCFP and the penult one. Sad to say, we were not able to make this part work finally. |
</p> | </p> | ||
<html> | <html> | ||
Revision as of 01:36, 12 October 2023
Pqitp
Promoter activated by VqmA in concert with dimethylpyrazin-2-ol (DPO).
Usage and Biology
We assembled Pqtip with BBa_K4645004then transfected into E. coli to sensing the density of bacteria and regulate the circuit.
Characterization
We inserted cCFP reporter gene behind qitp promoter to verify whether qtip promoter could work as expected in E. coli BL21(DE3). However, bacteria that have transformed this plasmid never shown any fluoresce, even though the sequence didn’t mutate. Later, we found that the sequence of qtip promoter contains more than one initiation codon. This may lead to the losing of efficiency of ECFP. So, we tried to delete sequence between eCFP and the last initiation codon and between eCFP and the penult one. Sad to say, we were not able to make this part work finally.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]