Difference between revisions of "Part:BBa K283011"

 
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<partinfo>BBa_K283011 short</partinfo>
 
<partinfo>BBa_K283011 short</partinfo>
  
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The fusion protein omp(46-159)streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The omp domain directs the expression of the fusion protein on the surface membrane
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The fusion protein lpp-ompA(46-159)-streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The backbone of this biobrick is based on BBa_J61003.lpp_ompA(46-159)_streptavidin(WT)_his tag lies between XbaI and SpeI.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:42, 20 October 2009

pTet-rbs-lpp_omp(46-159)_streptavidin_Histag

The fusion protein lpp-ompA(46-159)-streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The backbone of this biobrick is based on BBa_J61003.lpp_ompA(46-159)_streptavidin(WT)_his tag lies between XbaI and SpeI. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 607
    Illegal AgeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]