Difference between revisions of "Part:BBa K4989006:Design"

 
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===References===
 
===References===
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[1]“Roseburia Intestinalis L1-82 Chromosome 1, Complete Sequence.” NCBI Nucleotide, Mar. 2023, www.ncbi.nlm.nih.gov/nuccore/NZ_LR027880.1?report=genbank&from=430426&to=431769&strand=true. Accessed 12 Oct. 2023.
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Revision as of 00:59, 12 October 2023


Butyryl-CoA:Acetyl-CoA transferase (ButCoaT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 714
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence that we designed has a start and ending codon and a single open reading frame (ORF). Also, the sequence is expected to be expressed in our hosts Lactobacillus rhamnosus GG, Lactobacillus plantarum LP-01, Lactobacillus acidophilus DSM 20079 and E.coli K-12 and thus was codon-optimized.


Source

Our part was synthetically produced but the sequence comes from Roseburia intestinalis L1-82.

References

[1]“Roseburia Intestinalis L1-82 Chromosome 1, Complete Sequence.” NCBI Nucleotide, Mar. 2023, www.ncbi.nlm.nih.gov/nuccore/NZ_LR027880.1?report=genbank&from=430426&to=431769&strand=true. Accessed 12 Oct. 2023.