Difference between revisions of "Part:BBa K4645002:Design"
 
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===Design Notes===
 
===Design Notes===
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On the basis of the expression of functional target proteins, 6*His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N terminal of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.
+
On the basis of the expression of functional target proteins, His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N-terminus of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.
  
  
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===Source===
 
===Source===
  
−
Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. The amino acid sequence of one of the most effective monoclonal antibodies REGN1909 was obtained through literature review.
+
Velocimmune mice were immunized with recombinant dimeric Fel d 1 and produced polyclonal antibodies. The amino acid sequence of one of the most effective monoclonal antibodies REGN1909 was obtained through literature review.
  
 
===References===
 
===References===

Latest revision as of 00:30, 12 October 2023


NeuB: scFv ( single-chain fragment variable）that inhibits the immune response to the feline


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 679
    Illegal XhoI site found at 276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the basis of the expression of functional target proteins, His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N-terminus of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.


Source

Velocimmune mice were immunized with recombinant dimeric Fel d 1 and produced polyclonal antibodies. The amino acid sequence of one of the most effective monoclonal antibodies REGN1909 was obtained through literature review.

References