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Latest revision as of 00:30, 12 October 2023


RecA(K3)-B0032-eGFP

To optimize the design of the RecA-eGFP reporter (BBa_K4814015), we replaced the original B0034 RBS with RBS BBa_B0032, and see if the effectiveness will increase. However, from our experimental result, using RBS BBa_K4814002 does not enhance the level of fluorescence of the reporter.

RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.

RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 882
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 840
  • 1000
    COMPATIBLE WITH RFC[1000]