Difference between revisions of "Part:BBa K4837001:Design"
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| + | The biobricks contained in our plasmid are illustrated in the above graph. Two basic parts we designed, including BBa_K4837000 and BBa_K4837001 are used to synthesize lignin peroxidase (LiP) and manganese peroxidase (MnP) respectively. | ||
pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized. | pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized. | ||
Other biobricks such as BBa_K4837000 are required to perform its function. | Other biobricks such as BBa_K4837000 are required to perform its function. | ||
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===Source=== | ===Source=== | ||
Revision as of 00:20, 12 October 2023
Manganese peroxidase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 126
Illegal NotI site found at 781 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 66
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1149
Illegal NgoMIV site found at 1153
Illegal AgeI site found at 651
Illegal AgeI site found at 1132 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 865
Illegal SapI.rc site found at 523
Design Notes
The biobricks contained in our plasmid are illustrated in the above graph. Two basic parts we designed, including BBa_K4837000 and BBa_K4837001 are used to synthesize lignin peroxidase (LiP) and manganese peroxidase (MnP) respectively.
pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.
Other biobricks such as BBa_K4837000 are required to perform its function.
Source
It is designed based on part of white-rot fungus (Phanerochaete chrysosporium).