Difference between revisions of "Part:BBa K3002017:Experience"
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<p>We detected the expression of the POR with HA-tag via immunoblotting.</p> | <p>We detected the expression of the POR with HA-tag via immunoblotting.</p> | ||
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<p>For detection the UVM4 strain was transformed with the construct in (a). 30 hygromycin-resistant transformants were cultivated in TAP medium and samples were taken after 3 days. Whole-cell proteins were extracted and analyzed by SDS-PAGE and immunoblotting using an anti-HA antibody. The expression of the POR (~ 77 kDa) is visible. | <p>For detection the UVM4 strain was transformed with the construct in (a). 30 hygromycin-resistant transformants were cultivated in TAP medium and samples were taken after 3 days. Whole-cell proteins were extracted and analyzed by SDS-PAGE and immunoblotting using an anti-HA antibody. The expression of the POR (~ 77 kDa) is visible. | ||
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Revision as of 23:59, 11 October 2023
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