Difference between revisions of "Part:BBa K283002"

Revision as of 12:13, 20 October 2009

Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)

The speed control device was designed to control the gene expression of cheZ_his tag by using anhydrotetracycline or tetracycline.In the presence of anhydrotetracycline or tetracycline, which binds to TetR and remove its inhibiting effect on pTet promoter, the chez-histag(BBa_K283008) protein can be expressed. Consequently, the expression level of chez-histag,which responsible for the dephosphorylation of cheY protein in bacteria flagella movement, should be controlled by the concentration of anhydrotetracycline or tetracycline.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1782
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K283002 short</partinfo>
-This is a device that enable the control of chez(histag) using anhydrotetracycline or tetracycline. The pLacI promoter(BBa_R0010)enables high level of expression of the TetR repressor(BBa_C0040) protein.Without the presence of LacI repressor molecule, the pLacI promoter is constitutively on to express the TetR repressor protein.Therefore the ptet(BBa_R0040) promoter is repressed under normal condition. However, in the presence of anhydrotetracycline or tetracycline, which binds to TetR and remove its inhibiting effect on pTet promoter, the chez-histag(BBa_K283008) protein can be expressed. Consequently, the expression level of chez-histag,which responsible for the dephosphorylation of cheY protein in bacteria flagella movement, should be controlled by the concentration of anhydrotetracycline or tetracycline.
+The speed control device was designed to control the gene expression of cheZ_his tag by using anhydrotetracycline or tetracycline.In the presence of anhydrotetracycline or tetracycline, which binds to TetR and remove its inhibiting effect on pTet promoter, the chez-histag(BBa_K283008) protein can be expressed. Consequently, the expression level of chez-histag,which responsible for the dephosphorylation of cheY protein in bacteria flagella movement, should be controlled by the concentration of anhydrotetracycline or tetracycline.
 <!-- Add more about the biology of this part here