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Revision as of 23:46, 11 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4630114
This part is the more integrated form that contains three cassettes. Using this part, we can verify the concept of multi-level recording and gain comprehensive data.
General Induction Protocol
- Pick a colony from the verified plate, cultivate in 5ml liquid LB media for 12 hours.
- Transfer 100µl bacteria solution to 3ml new liquid LB media. Cultivate to an OD600 of 0.3~0.5.
- Add L-Arabinose and IPTG to final concentrations of 2g/L and 3g/L, and induce for 22 hours and 5 hours, respectively.
- Spread the diluted solution to plates with appropriate antibiotics and cultivate for 12 hours.
- Pick the colonies and test the editing result.
Result
Proof-of-Concept
We implement induction over the part, and it is successfully knock-out.
Fig 1 The successful knock-out of the stgRNA-cassette (1+2+3)
Also, we performed time-gradient tests over the part.
Fig 2 Time-gradient test over the part
User Reviews
UNIQbecee7d1929e170a-partinfo-00000002-QINU UNIQbecee7d1929e170a-partinfo-00000003-QINU