Difference between revisions of "Part:BBa K228258"
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{{PKU_Beijing2009_AND_GATE_FAMILY}} | {{PKU_Beijing2009_AND_GATE_FAMILY}} | ||
| − | + | We have adapted the original AND gate designed by Christopher A Voigt, which is published on Molecular Systems Biology, as the first module of our entire logic system. As the input module, the AND gate accepts the small molecules as inputs and integrate the signals to activate the T7 promoter, thus triggering the expression of downstream gene. | |
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| + | [[Image:PKU_AND_Gate_Mechanism.png|600px|center|thumb|fig1. '''The mechanism of the AND Gate''' J Christopher Anderson, Christopher A Voigt and Adam P Arkin (2007) ''Environmental signal integration by a modular AND gate'' '''Molecular Systems Biology 3:133''']] | ||
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Use arabinose to induce the transcription of SupD and use salicylate to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter. | Use arabinose to induce the transcription of SupD and use salicylate to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter. | ||
Revision as of 11:45, 20 October 2009
AND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag
This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team
We have adapted the original AND gate designed by Christopher A Voigt, which is published on Molecular Systems Biology, as the first module of our entire logic system. As the input module, the AND gate accepts the small molecules as inputs and integrate the signals to activate the T7 promoter, thus triggering the expression of downstream gene.
Use arabinose to induce the transcription of SupD and use salicylate to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
This gate is only one of nine gates(K228255-K228263), which are different in the RBS of T7ptag.
For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1274
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2144
Illegal BamHI site found at 1214 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1976
Illegal AgeI site found at 1320 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1323