Difference between revisions of "Part:BBa K4855003:Design"

 
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===Design Notes===
 
===Design Notes===
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Assembled following the rules of the BioBrick RFC10 assembly procedure from parts J23101 and BBa_K4855002.  
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Assembled following the rules of the BioBrick RFC10 assembly procedure from parts J23101 and BBa_K4855002. Dual promoters: J23101 for screening and T7 for mutagenesis.
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===Source===
 
===Source===

Latest revision as of 23:23, 11 October 2023


J23101 - T7 - medium RBS - blaNDM2&5 CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembled following the rules of the BioBrick RFC10 assembly procedure from parts J23101 and BBa_K4855002. Dual promoters: J23101 for screening and T7 for mutagenesis.

Source

This part was designed to be cloned in low copy number vectors and used with the dual gene-specific mutator system from Addgene (doi: 10.1093/nar/gkad266)

References