Difference between revisions of "Part:BBa K4630114"
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StgRNA-cassette (1+2) is the ligated form of stgRNA-cassette (1+2) and stgRNA-cassette 3. It construct the multi-level recorder allowing the biological events to trigger recording three times. | StgRNA-cassette (1+2) is the ligated form of stgRNA-cassette (1+2) and stgRNA-cassette 3. It construct the multi-level recorder allowing the biological events to trigger recording three times. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
We integrate the two methods into a single cassette, with unique barcode (fig B2). Once triggered, double strand break (DSB) is introduced into the working plasmid, enabling the intra-plasmid recombination. It is worth noting that the two homologous arms flank the stgRNA, so the entire cassette will be deleted after the recombination, and the next cassette is expected to be brought to the proper expressing place. The inducer triggers over and over, and recorder records it by keeping changing the cassette. | We integrate the two methods into a single cassette, with unique barcode (fig B2). Once triggered, double strand break (DSB) is introduced into the working plasmid, enabling the intra-plasmid recombination. It is worth noting that the two homologous arms flank the stgRNA, so the entire cassette will be deleted after the recombination, and the next cassette is expected to be brought to the proper expressing place. The inducer triggers over and over, and recorder records it by keeping changing the cassette. | ||
Revision as of 22:46, 11 October 2023
stgRNA-cassette (1+2+3)
StgRNA-cassette (1+2) is the ligated form of stgRNA-cassette (1+2) and stgRNA-cassette 3. It construct the multi-level recorder allowing the biological events to trigger recording three times.
Usage and Biology
We integrate the two methods into a single cassette, with unique barcode (fig B2). Once triggered, double strand break (DSB) is introduced into the working plasmid, enabling the intra-plasmid recombination. It is worth noting that the two homologous arms flank the stgRNA, so the entire cassette will be deleted after the recombination, and the next cassette is expected to be brought to the proper expressing place. The inducer triggers over and over, and recorder records it by keeping changing the cassette.
Figure B2. Working pattern of the change of cassette
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]