Difference between revisions of "Part:BBa K4604021"
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BioBrick piG_10 consists of the tet promoter/repressor, bluB, a riboswitch for AdoCbl, MazF and the rrnB terminator. The backbone we used in the experiments is pGGAselect. The tet promoter is a BioBrick from iGEM Freiburg 2022 (<a href="https://parts.igem.org/Part:BBa_K4229059">BBa_K4229059</a>).</html>
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BioBrick piG_10a consists of the tet promoter/repressor, the modified T7 RBS, <i>bluB</i>, a riboswitch for AdoCbl, <i>mazF</i> and the rrnB terminator. The backbone we used in the experiments is pGGAselect. The tet promoter is a BioBrick from iGEM Freiburg 2022 (<a href="https://parts.igem.org/Part:BBa_K4229059">BBa_K4229059</a>).</html>
  
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===Characterization===
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===Usage and Biology===
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This BioBrick is the closest we got to testing all the parts our system consists of combined. Read more about the full setup on <a href="https://parts.igem.org/Part:BBa_K4604023">Ba_K4604023</a>. </html>
  
 
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Revision as of 22:46, 11 October 2023


piG_10a (tetR_bluB_riboK12_mazF)

BioBrick piG_10a consists of the tet promoter/repressor, the modified T7 RBS, bluB, a riboswitch for AdoCbl, mazF and the rrnB terminator. The backbone we used in the experiments is pGGAselect. The tet promoter is a BioBrick from iGEM Freiburg 2022 (BBa_K4229059).

Characterization

This BioBrick is the closest we got to testing all the parts our system consists of combined. Read more about the full setup on Ba_K4604023.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2238