Difference between revisions of "Part:BBa K4757062"
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Engnineering journey
 
Engnineering journey
 
<p>Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected </p>
 
<p>Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected </p>
−
 
+
<p> co-induction showed so low exprssion -> pEM7 promoter was used to substitute Ps1/Ps2 -> high expression but leaky </p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:39, 11 October 2023


TPA sensing XylS-K38R-L224Q/Pm expressing mKate2

Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette..... to be continued...

Engnineering journey

Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected

co-induction showed so low exprssion -> pEM7 promoter was used to substitute Ps1/Ps2 -> high expression but leaky

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 934
  • 1000
    COMPATIBLE WITH RFC[1000]