Difference between revisions of "Part:BBa K4757062"
| Line 5: | Line 5: | ||
Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette..... | Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette..... | ||
to be continued... | to be continued... | ||
| + | |||
| + | Engnineering journey | ||
| + | <p>Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected </p> | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 22:37, 11 October 2023
TPA sensing XylS-K38R-L224Q/Pm expressing mKate2
Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette..... to be continued...
Engnineering journey
Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 212
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 934
- 1000COMPATIBLE WITH RFC[1000]