Difference between revisions of "Part:BBa K4830017:Design"

(Source)
(References)
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===References===
 
===References===
 +
López-González, M.J. et al. (2018) ‘Adaptive evolution of Industrial Lactococcus lactis under cell envelope stress provides phenotypic diversity’, Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.02654.

Revision as of 22:23, 11 October 2023


Ll.ltrA RT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence of the protein was not altered in anyway. In order to use it for prime editing, this part has been fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.

Source

NCBI ACCESSION: RQG78521

References

López-González, M.J. et al. (2018) ‘Adaptive evolution of Industrial Lactococcus lactis under cell envelope stress provides phenotypic diversity’, Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.02654.