Difference between revisions of "Part:BBa K4788002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The front of this part requires a promoter to express the FadR protein in order to achieve oleic acid response function. Therefore, in practical use, appropriate promoters or plasmid skeletons should be selected. | |
− | + | In longer-term applications, we can try to change the number and position of FadO operators to change the oleic acid induction threshold to achieve more precise promoter regulation. | |
− | + | ||
===Source=== | ===Source=== |
Revision as of 21:52, 11 October 2023
Oleic acid inducer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 764
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The front of this part requires a promoter to express the FadR protein in order to achieve oleic acid response function. Therefore, in practical use, appropriate promoters or plasmid skeletons should be selected. In longer-term applications, we can try to change the number and position of FadO operators to change the oleic acid induction threshold to achieve more precise promoter regulation.
Source
xx