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Revision as of 21:07, 11 October 2023


PFV RT

Prototype Foamy virus Reverse transcriptase.

Usage and Biology

PFV RT retrieved from chimpanzee was human codon optimized and fused to nickase Cas9 to form prime editor.

Characterization

It was tested on endogeneous sites as well TLR reporter using HEK293T cells to evaluate the alternative RT performance in terms of its ability to introduce different edits on different target sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]