Difference between revisions of "Part:BBa K203108"
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Localization sequence to direct a protein into the cell membrane. This part has the BBb-standard restriction sites. | Localization sequence to direct a protein into the cell membrane. This part has the BBb-standard restriction sites. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K203108 parameters</partinfo> | <partinfo>BBa_K203108 parameters</partinfo> | ||
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| + | Shown to localize proteins to the ER. An expression device consisting of [[Part:BBa_I712004]] (CMV) [[Part:BBa_K203103]] (GFP), [[Part:BBa_K203108]] and [[Part:BBa_K203102]] (terminator) was assembled for this characterization. | ||
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| + | [[Image:Myrpalm.jpg]] | ||
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Revision as of 09:22, 20 October 2009
Plasma membrane targeting (Myrpalm)
Localization sequence to direct a protein into the cell membrane. This part has the BBb-standard restriction sites.
Usage and Biology
This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 86
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 49
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 46
Functional Parameters
| n/a | Plasma membrane targeting (Myrpalm) |
Shown to localize proteins to the ER. An expression device consisting of Part:BBa_I712004 (CMV) Part:BBa_K203103 (GFP), Part:BBa_K203108 and Part:BBa_K203102 (terminator) was assembled for this characterization.
