Difference between revisions of "Part:BBa K203101"
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Shown to localize proteins to the ER. An expression device consisting of [[Part:BBa_I712004]] (CMV) [[Part:BBa_K203103]] (GFP), [[Part:BBa_K203101]] (Sar-1) and [[Part:BBa_K203102]] (terminator) was assembled for this characterization. | Shown to localize proteins to the ER. An expression device consisting of [[Part:BBa_I712004]] (CMV) [[Part:BBa_K203103]] (GFP), [[Part:BBa_K203101]] (Sar-1) and [[Part:BBa_K203102]] (terminator) was assembled for this characterization. | ||
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[[Image:ER2.jpg]] | [[Image:ER2.jpg]] | ||
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Latest revision as of 08:45, 20 October 2009
ER membrane targeting (C-terminal)
This part is a protein domain of the Sar-1 GTPase known to localize to the ER membrane. It is very convenient as a localization signal to the ER for proteins to which it is fused.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 133
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 133
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 133
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| n/a | ER membrane targeting (C-terminal) |
Shown to localize proteins to the ER. An expression device consisting of Part:BBa_I712004 (CMV) Part:BBa_K203103 (GFP), Part:BBa_K203101 (Sar-1) and Part:BBa_K203102 (terminator) was assembled for this characterization.
