Difference between revisions of "Part:BBa K2549044"
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CD8&#945; signal peptide guides synthesized fusion protein to pass the translocon<ref>https://en.wikipedia.org/wiki/Translocon</ref> into the endoplasmic reticulum<ref>https://en.wikipedia.org/wiki/Endoplasmic_reticulum</ref>, and the fusion protein will be later sugar modified in Golgi<ref>https://en.wikipedia.org/wiki/Golgi_apparatus</ref>, presented on the plasma membrane and located to the outside of the cell. We include this part as it is required for the parts collection [[Part:BBa_K2549016]] ~ [[Part:BBa_K2549043]].
 
CD8&#945; signal peptide guides synthesized fusion protein to pass the translocon<ref>https://en.wikipedia.org/wiki/Translocon</ref> into the endoplasmic reticulum<ref>https://en.wikipedia.org/wiki/Endoplasmic_reticulum</ref>, and the fusion protein will be later sugar modified in Golgi<ref>https://en.wikipedia.org/wiki/Golgi_apparatus</ref>, presented on the plasma membrane and located to the outside of the cell. We include this part as it is required for the parts collection [[Part:BBa_K2549016]] ~ [[Part:BBa_K2549043]].
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==Literature Characterization by AFCM-Egypt==
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The study created a reporter construct by joining the C-terminus of CD63, one of the most used exosome markers, to nanoluc (nluc), a tiny and potent bioluminescence reporter10. After progressive centrifugation to eliminate masking signals12, luminescence in the cell-culture supernatant was measured. This reporter gene was co-transfected with plasmids expressing potential candidates for exosome production augmentation.
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<html><div align="center"style="border:solid #17252A; width:50%;float:center;"><img style="                              max-width:850px;
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"src="https://static.igem.wiki/teams/4586/wiki/literature-characterisation-parts/cd8-alpha.png">
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Poly(A) RNA samples from each cell line were separated by gel electrophoresis, transferred to a nitrocellulose membrane, and hybridized with 32P-labeled cDNA probes specific for MC-CPA or CD8amp;. The membranes were then reprobed with a 32P-labeled p-actin cDNA probe to verify that equal amounts of RNA had been loaded in each lane.
  
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The results showed that MC-CPA mRNA was expressed at a higher level in MW9.11-3 cells than in MC/9.IL-4 cells, while CDalpha mRNA was expressed at a similar level in both cell lines. These findings suggest that MC-CPA and CDalpha may play different roles in the development and differentiation of these two cell
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:48, 11 October 2023


CD8alpha signal peptide

CD8α signal peptide guides synthesized fusion protein to pass the translocon[1] into the endoplasmic reticulum[2], and the fusion protein will be later sugar modified in Golgi[3], presented on the plasma membrane and located to the outside of the cell. We include this part as it is required for the parts collection Part:BBa_K2549016 ~ Part:BBa_K2549043.

Literature Characterization by AFCM-Egypt

The study created a reporter construct by joining the C-terminus of CD63, one of the most used exosome markers, to nanoluc (nluc), a tiny and potent bioluminescence reporter10. After progressive centrifugation to eliminate masking signals12, luminescence in the cell-culture supernatant was measured. This reporter gene was co-transfected with plasmids expressing potential candidates for exosome production augmentation.

Poly(A) RNA samples from each cell line were separated by gel electrophoresis, transferred to a nitrocellulose membrane, and hybridized with 32P-labeled cDNA probes specific for MC-CPA or CD8amp;. The membranes were then reprobed with a 32P-labeled p-actin cDNA probe to verify that equal amounts of RNA had been loaded in each lane. The results showed that MC-CPA mRNA was expressed at a higher level in MW9.11-3 cells than in MC/9.IL-4 cells, while CDalpha mRNA was expressed at a similar level in both cell lines. These findings suggest that MC-CPA and CDalpha may play different roles in the development and differentiation of these two cell

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ https://en.wikipedia.org/wiki/Translocon
  2. ↑ https://en.wikipedia.org/wiki/Endoplasmic_reticulum
  3. ↑ https://en.wikipedia.org/wiki/Golgi_apparatus