Difference between revisions of "Part:BBa K4601000"

Latest revision as of 18:58, 11 October 2023

CatCh

This part is the E. coli codon optimised version of CatCh channelrhodopsin.

Usage and Biology

CatCh is a channelrhodopsin derived from the ChR2 of Chlamydomonas reinhardtii [1], from which it only retains the first 309 amino acids containing the N-terminal transmembrane domain forming. Compared to ChR2, it also contains one mutation L132C that modifies the photocurrent generated upon light irradiation. CatCh is a Ca2+ inward pump that is also capable of transporting, although to a lesser extent, Na⁺, K⁺ and Mg²⁺. It was successfully used in optogenetic therapies for visual restoration in primates [2].

For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601200.

References

[1] Kleinlogel S, Feldbauer K, Dempski RE, Fotis H, Wood PG, Bamann C, Bamberg E. Ultra light-sensitive and fast neuronal activation with the Ca2+-permeable channelrhodopsin CatCh. Nature Neuroscience (2011) 14: 513–518.

[2] Chaffiol A, Caplette R, Jaillard C, Brazhnikova E, Desrosiers M, Dubus E, Duhamel L, Macé E, Marre O, Benoit P, Hantraye P, Bemelmans A-P, Bamberg E, Duebel J, Sahel J-A, Picaud S, Dalkara D. A new promoter allows optogenetic vision restoration with enhanced sensitivity in macaque retina. Molecular Therapy: The Journal of the American Society of Gene Therapy (2017) 25: 2546–2560.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 623
Illegal AgeI site found at 508
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4601000 short</partinfo>
-This part is the ''E. coli'' codon optimized version of CatCh channelrhodopsin.
+This part is the ''E. coli'' codon optimised version of CatCh channelrhodopsin.
 ===Usage and Biology===
 CatCh is a channelrhodopsin derived from the ChR2 of ''Chlamydomonas reinhardtii'' [1], from which it only retains the first 309 amino acids containing the N-terminal transmembrane domain forming. Compared to ChR2, it also contains one mutation L132C that modifies the photocurrent generated upon light irradiation. CatCh is a Ca2+ inward pump that is also capable of transporting, although to a lesser extent, Na<sup>+</sup>, K<sup>+</sup> and Mg<sup>2+</sup>. It was successfully used in optogenetic therapies for visual restoration in primates [2].
+For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: [[Part:BBa_K4601200|BBa_K4601200]].
 ===References===