Difference between revisions of "Part:BBa K260010"
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| − | This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology arms and a codon-optimised blasticidin resistance cassette (BsdR) for insertion of the contained BioBrick into the proper locus of pCC2FosM ([[Part:BBa_K260000|BBa_K260000]]) by recombineering using the HincII fragment including the BioBrick. The product of this reaction is the pCC2FosMB backbone ([[Part:BBa_K260001|BBa_K260001]]), which then contains the same BioBrick that was inside the present backbone [[Part:BBa_K260010|BBa_K260010]] before, with BioBrick prefix and sufix maintained intact. Initially this was done with the TT_FRT_GFP BioBrick ([[Part:BBa_K260016|BBa_K260016]]) using [[Part:BBa_K260010|BBa_K260010]]-[[Part:BBa_K260016|BBa_K260016]]. | + | This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology arms and a codon-optimised blasticidin resistance cassette (BsdR) for insertion of the contained BioBrick into the proper locus of pCC2FosM ([[Part:BBa_K260000|BBa_K260000]]) by recombineering using the HincII fragment including the BioBrick. The product of this reaction is the pCC2FosMB backbone ([[Part:BBa_K260001|BBa_K260001]]), which then contains the same BioBrick that was inside the present backbone [[Part:BBa_K260010|BBa_K260010]] before, with BioBrick prefix and sufix maintained intact. Initially this was done with the TT_FRT_GFP BioBrick ([[Part:BBa_K260016|BBa_K260016]]), which is depicted below, using [[Part:BBa_K260010|BBa_K260010]]-[[Part:BBa_K260016|BBa_K260016]]. |
| + | [[Image:pMA-TT_FRT_GFP_BsdR.jpg]] | ||
Latest revision as of 06:14, 20 October 2009
pMA-BsdR-@CC2FosM
DESCRIPTION:
This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology arms and a codon-optimised blasticidin resistance cassette (BsdR) for insertion of the contained BioBrick into the proper locus of pCC2FosM (BBa_K260000) by recombineering using the HincII fragment including the BioBrick. The product of this reaction is the pCC2FosMB backbone (BBa_K260001), which then contains the same BioBrick that was inside the present backbone BBa_K260010 before, with BioBrick prefix and sufix maintained intact. Initially this was done with the TT_FRT_GFP BioBrick (BBa_K260016), which is depicted below, using BBa_K260010-BBa_K260016.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3076
Illegal NheI site found at 3014
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3082 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3076 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3076
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3076
Plasmid lacks a suffix.
Illegal XbaI site found at 3091
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.