Difference between revisions of "Part:BBa K260009"
 
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This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP ([[Part:BBa_K260017|BBa_K260017]]) depicted below. The product of the above-described recombineering reaction [[Part:BBa_K260002|BBa_K260002]]-[[Part:BBa_K260017|BBa_K260017]] is also available.
 
This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP ([[Part:BBa_K260017|BBa_K260017]]) depicted below. The product of the above-described recombineering reaction [[Part:BBa_K260002|BBa_K260002]]-[[Part:BBa_K260017|BBa_K260017]] is also available.
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[[Image:pMA-RQ-P_F3_zeoR_F3_RFP.jpg]]
 
[[Image:pMA-RQ-P_F3_zeoR_F3_RFP.jpg]]
  

Latest revision as of 06:13, 20 October 2009

pMA-RQ-@TetFlp

This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology to pTetFlp (BBa_K260002) for tranfer of the contained BioBrick part by recombineering, for which the BioBrick needs to be excised with PvuII from the backbone.

This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP (BBa_K260017) depicted below. The product of the above-described recombineering reaction BBa_K260002-BBa_K260017 is also available.

PMA-RQ-P F3 zeoR F3 RFP.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2578
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2584
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2578
    Illegal XhoI site found at 61
    Illegal XhoI site found at 2572
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2578
    Illegal suffix found at 2
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2578
    Plasmid lacks a suffix.
    Illegal XbaI site found at 67
    Illegal XbaI site found at 2593
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.