Difference between revisions of "Part:BBa K4716009"

 
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<partinfo>BBa_K4716009 short</partinfo>
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On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) is the ribosome binding site, the target gene - CsaA and mSandy2, Cp19k are connected with the sequence of GS linker, and the mSandy2 is the reporter gene; The double terminator (BBa_B0015) is the terminater we use in this circuit, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene, respectively. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4716009 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4716009 parameters</partinfo>
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Revision as of 18:04, 11 October 2023


pSB1C3-Pasr-CsgA-cp19k-mSandy

On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) is the ribosome binding site, the target gene - CsaA and mSandy2, Cp19k are connected with the sequence of GS linker, and the mSandy2 is the reporter gene; The double terminator (BBa_B0015) is the terminater we use in this circuit, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene, respectively. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]