Difference between revisions of "Part:BBa K4759044:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
linker
+
 
  
 
===References===
 
===References===

Latest revision as of 18:03, 11 October 2023


RBM3-linker-GFP1-10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 385
    Illegal BsaI.rc site found at 1309
    Illegal SapI.rc site found at 865
    Illegal SapI.rc site found at 1465


Design Notes

Connecting GFP1-10 to the C terminus of RBM3 by linker and GFP11 to OleP, and the interacting protein will bring GFP11 to GFP1-10 close to the fluorescence, the stronger the interaction, we screened the two proteins with the strongest interaction force by bimolecular fluorescence.


Source

References