Difference between revisions of "Part:BBa K4759269:Design"
(→‎Design Notes)
 
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We cloned <i>pntAB</i> gene to construct the plasmid pACYCDuet-pntAB
 
We cloned <i>pntAB</i> gene to construct the plasmid pACYCDuet-pntAB
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===Source===
 
===Source===

Latest revision as of 17:45, 11 October 2023


T7-RBS6-pntAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal XhoI site found at 720
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal NgoMIV site found at 1190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned pntAB gene to construct the plasmid pACYCDuet-pntAB

Source

References