Difference between revisions of "Part:BBa K4716012"

 
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On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) which is the ribosome binding site, mSandy gene is our fluorescent reporter gene, double terminator
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(BBa_B0015) is the terminator, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid. This plasmid is the design for validating whether or not the new fluorescent reporter gene mSandy2 we use has a good RFP property in our
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project replace the mCherry part of BBa_K4357004 (pSB1C3-Pasr-mCherry).

Revision as of 17:38, 11 October 2023

On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) which is the ribosome binding site, mSandy gene is our fluorescent reporter gene, double terminator (BBa_B0015) is the terminator, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid. This plasmid is the design for validating whether or not the new fluorescent reporter gene mSandy2 we use has a good RFP property in our project replace the mCherry part of BBa_K4357004 (pSB1C3-Pasr-mCherry).