Difference between revisions of "Part:BBa K4759271:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We cloned <i>nadK</i> and <i>pntAB</i> genes to construct the plasmid pACYCDuet-pntAB-nadK.
  
  
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===Source===
 
===Source===
  
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===References===
 
===References===

Latest revision as of 17:36, 11 October 2023


T7-RBS6-pntAB-RBS2-nadK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal EcoRI site found at 1626
    Illegal PstI site found at 2233
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal EcoRI site found at 1626
    Illegal PstI site found at 2233
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal EcoRI site found at 1626
    Illegal BglII site found at 2158
    Illegal BamHI site found at 1620
    Illegal XhoI site found at 720
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal EcoRI site found at 1626
    Illegal PstI site found at 2233
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 714
    Illegal EcoRI site found at 1353
    Illegal EcoRI site found at 1626
    Illegal PstI site found at 2233
    Illegal NgoMIV site found at 1190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned nadK and pntAB genes to construct the plasmid pACYCDuet-pntAB-nadK.


Source

References