Difference between revisions of "Part:BBa K4830028:Design"
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===Design Notes=== | ===Design Notes=== | ||
A premature stop codon has to be installed into a functional eGFP. | A premature stop codon has to be installed into a functional eGFP. | ||
| − | + | The premature stop codon requires only a simple edit (two nucleotides change) to restore the wild-type eGFP. | |
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 17:34, 11 October 2023
eGFP_simple premature stop codon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A premature stop codon has to be installed into a functional eGFP. The premature stop codon requires only a simple edit (two nucleotides change) to restore the wild-type eGFP.
Source
The eGFP is derived from Aequorea victoria. It has an excitation maxima of 488nm, and emission maxima of 509nm.
References
Certo, M. T., Ryu, B. Y., Annis, J. E., Garibov, M., Jarjour, J., Rawlings, D. J., & Scharenberg, A. M. (2011). Tracking genome engineering outcome at individual DNA breakpoints. Nature Methods, 8(8), 671–676.