Difference between revisions of "Part:BBa K4779000"

Revision as of 17:23, 11 October 2023

CMPG:A yeast copper-induced reporter pathway with prm1 promoter

This is a copper ion-responsive signaling pathway in Saccharomyces cerevisiae based on MAPK system. When the cells were induced by copper ions, the circuit with prm1 promoter activates GFP expression to indicate the copper ion concentration.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 279
Illegal XbaI site found at 517
Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 55
Illegal BamHI site found at 510
Illegal XhoI site found at 8
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 279
Illegal XbaI site found at 517
Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 279
Illegal XbaI site found at 517
Illegal SpeI site found at 343
Illegal SpeI site found at 523
Illegal PstI site found at 49
Illegal NgoMIV site found at 36
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2213
Illegal SapI site found at 529

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 This is a copper ion-responsive signaling pathway in Saccharomyces cerevisiae based on MAPK system. When the cells were induced by copper ions, the circuit with prm1 promoter activates GFP expression to indicate the copper ion concentration.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===