Difference between revisions of "Part:BBa K4759042:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
gene
+
<i>E. coli</i> genome
  
 
===References===
 
===References===

Latest revision as of 17:21, 11 October 2023


hemB-RBS2-hemD-RBS2-hemC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 982
    Illegal EcoRI site found at 1759
    Illegal EcoRI site found at 2050
    Illegal PstI site found at 467
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 982
    Illegal EcoRI site found at 1759
    Illegal EcoRI site found at 2050
    Illegal PstI site found at 467
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 982
    Illegal EcoRI site found at 1759
    Illegal EcoRI site found at 2050
    Illegal BamHI site found at 976
    Illegal BamHI site found at 1753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 982
    Illegal EcoRI site found at 1759
    Illegal EcoRI site found at 2050
    Illegal PstI site found at 467
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 982
    Illegal EcoRI site found at 1759
    Illegal EcoRI site found at 2050
    Illegal PstI site found at 467
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2662


Design Notes

Connect the hemB, hemD, and hemC via RBS2 for transcription under the regulation of the same promoter


Source

E. coli genome

References