Difference between revisions of "Part:BBa K4601001"

Revision as of 17:03, 11 October 2023

ChrimsonR

This part is the E. coli codon optimized version of ChrimsonR channelrhodopsin.

Usage and Biology

ChrimsonR is a channelrhodopsin derived from the CR Chrimson of 'Chlamydomonas noctigama' that is naturally red-shifted [1]. Compared to the Chrimson, ChrimsonR contains one point mutation K176R that does not modify the absorption properties, but increases the photocurrent generated upon light irradiation. ChrimsonR is a Na+ inward pump that is also capable of transporting H⁺, K⁺ and Ca²⁺. It is one of the few microbial opsins in clinical trials for gene therapy for patients with documented diagnosis of non-syndromic Retinitis Pigmentosa [2].

References

[1] Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK-S, Boyden ES. Independent optical excitation of distinct neural populations. Nature Methods (2014) 11: 338–346.

[2] GenSight Biologics. A Phase 1/2a, Open-label, non-randomized, dose-eEscalation study to evaluate the safety and tolerability of GS030 in subjects with Retinitis Pigmentosa. clinicaltrials.gov (2022).

Sequence and Features