Difference between revisions of "Part:BBa K4586031"

Revision as of 16:59, 11 October 2023

ZF21.16 minCMV promoter - Cre recombinase

Usage and Description

This composite part is a translational unit implemented in our project for conditional expression of Cre recombinase enzyme following stimulation of vimentin syn notch receptor. This enzyme controls the conditional expression of our booster genes (SDC4, STEAP3, NadB) (BBa_K4586027) by deleting (STOP) sequence flanked by two loxP sites upstream to the coding of our booster genes.

This figure illustrates the role of the Cre loxP system in regulation the expression of our booster genes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 673

@@ Line 7: / Line 7: @@
 This composite part is a translational unit implemented in our project for conditional expression of Cre recombinase enzyme following stimulation of vimentin syn notch receptor.
 This enzyme controls the conditional expression of our booster genes (SDC4, STEAP3, NadB) (BBa_K4586027) by deleting (STOP) sequence flanked by two loxP sites upstream to the coding of our booster genes.
+<html><div align="center"style="border:solid #17252A; width:50%;float:center;"><img style="                              	max-width:850px;
+							width:75%;
+							height:auto;
+								position: relative;
+							top: 50%;
+							left: 35%;
+							transform: translate( -50%);
+							padding-bottom:25px;
+							padding-top:25px;
+							"src="https://static.igem.wiki/teams/4586/wiki/parts/pasted-image-0.png">
+<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
+lang=EN style='font-size:11.0pt;line-height:115%'>This figure illustrates the role of the Cre loxP system in regulation the expression of our booster genes.
+ </span></p></div></html>