Difference between revisions of "Part:BBa K4645015"
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| + | The pH-sensitive circuit can detect pH changes when engineered bacteria enter a cat's digestive tract and take effect, expressing CI protein to inhibit the expression of antitoxin protein MntA. At the same time, it expresses the two-component system RstAB. RstB can sense the decrease in pH to phosphorylate RstA, and RstA can increase the activity of the acid-induced promoter Pasr, thus quickly increasing the expression of CI protein. | ||
| + | The toxin protein HepT and antitoxin protein MntA form a toxin-antitoxin pathway, where the expression of MntA protein is inhibited by CI protein.The circuit design is shown in the diagram below. | ||
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| + | <center><img src="https://static.igem.wiki/teams/4645/wiki/wet-lab/suicide/ph.jpg" style="width:50%; "></center> | ||
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| + | <center><b>Figure 1. Acid-sensitive suicide and Toxin-antitoxin system.</b> </center> | ||
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Revision as of 16:36, 11 October 2023
pH-antitoxin system The pH-sensitive circuit can detect pH changes when engineered bacteria enter a cat's digestive tract and take effect, expressing CI protein to inhibit the expression of antitoxin protein MntA. At the same time, it expresses the two-component system RstAB. RstB can sense the decrease in pH to phosphorylate RstA, and RstA can increase the activity of the acid-induced promoter Pasr, thus quickly increasing the expression of CI protein. The toxin protein HepT and antitoxin protein MntA form a toxin-antitoxin pathway, where the expression of MntA protein is inhibited by CI protein.The circuit design is shown in the diagram below.
