Difference between revisions of "Part:BBa K4800013"
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SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the YcjQ SgRNA is derived from the YcjQ gene and can be localised to the acka-pta gene in the <i>Escherichia coli</i> NT1003 genome to assist the Cas9 protein in silencing the gene.
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SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the YcjQ SgRNA is derived from the YcjQ gene and can be localised to the YcjQ gene in the <i>Escherichia coli</i> NT1003 genome to assist the Cas9 protein in silencing the gene.
  
 
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Revision as of 15:32, 11 October 2023


YcjQ sgRNA

SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the YcjQ SgRNA is derived from the YcjQ gene and can be localised to the YcjQ gene in the Escherichia coli NT1003 genome to assist the Cas9 protein in silencing the gene.


Document

Build:

Fig.1 YcjQ sgRNA

We designed the N20 sequence of YcjQ by using the CHOPCHOP website (http://chopchop.cbu.uib.no), synthesized them on primers and ligated the N20 into the pTarget-sgRNA plasmid by means of PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]