Difference between revisions of "Part:BBa K4645014"

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<center><img src="https://static.igem.wiki/teams/4645/wiki/wet-lab/suicide/rnarele.png" style="width:50%; "></center>
 
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<center><b>Figure 1. Bacterial OD600 over time .</b> </center>
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Revision as of 15:32, 11 October 2023


Combination element of RNA thermosensors regulatory RelE toxin protein

RelE is a toxin protein that inhibits translation. RNA thermosensors which could inhibit downstream gene expression when the temperature is 37 ℃ but not affect downstream gene expression significantly when the temperature is below 28 ℃.The combination of two components can regulate the expression of toxin proteins by changing the temperature, and then control the death of engineering bacteria. 无标题文档



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 82