Difference between revisions of "Part:BBa K4960034"

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−	Figure 2.(a)Figure1.Schematic Diagram of Golden Gate Assembly Strategy for New pvc13-based pPVC Constructs<br>	+	Figure 2.(a)Schematic Diagram of Golden Gate Assembly Strategy for New pvc13-based pPVC Constructs<br>
	(b)Schematic diagram of the newly-designed pvc13 part allowing Golden Gate cloning of targeted sequence into the pPVC plasmid.<br>		(b)Schematic diagram of the newly-designed pvc13 part allowing Golden Gate cloning of targeted sequence into the pPVC plasmid.<br>

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Revision as of 14:51, 11 October 2023

pPVC promoter->pvc1-pvc12->pvc13_NTD-2*Bsal-pVc13_CTD->pvc14-pvc16

Structural and accessory genes

Usage and Biology

This part is necessary for the assembly of a functional injection system. PVCs probably recognize target cells via tail fibres (Pvc13), leading to a contraction of the sheath mechanism that drives a spike through the cellular membrane.[1]
Pvc2, Pvc3 and Pvc4 compose the sheath of PVC. Pvc16 is sheath terminator of PVC. Pvc13 is tail fibres of PVC. Pvc9, Pvc11 and Pvc12 compose the baseplate of PVC. Pvc8 and Pvc10 compose the spike of PVC. Pvc1, Pvc5 and Pvc7 compose the tube of PVC.[2]（Fig.1)
This part has a Bsal enzyme recognizing sequence, which can allow to use the way Golden Gate Assembly to construct a plasmid.(Fig.2(a),Fig2(b))

Figure 1.PVC composition diagram
(a)

(b)

Figure 2.(a)Schematic Diagram of Golden Gate Assembly Strategy for New pvc13-based pPVC Constructs
(b)Schematic diagram of the newly-designed pvc13 part allowing Golden Gate cloning of targeted sequence into the pPVC plasmid.

Special Design

This part has a Bsal enzyme recognizing sequence, which can allow to use the way Golden Gate Assembly to construct a plasmid.

Sequence and Features

Tube:pvc1, pvc5, pvc7
Sheath:pvc2, pvc3, pvc4
Sheath terminator:pvc15
Tail fbres:pvc13_NTD-2*Bsal-pVc13_CTD
Baseplate:pvc9, pvc11, pvc12
Spike:pvc8, pvc10
Assembly method:3A Assembly

References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr.
[2]Kolonin MG, Saha PK, Chan L, Pasqualini R, Arap W. Reversal of obesity by targeted ablation of adipose tissue. Nat Med. 2004 Jun.