Difference between revisions of "Part:BBa K4830019"

Revision as of 14:29, 11 October 2023

TLR pegRNA 1

TLR pegRNA 1 - prime editing guide RNA (pegRNA) targeting the premature stop codon instilled in a mCherry fluorescent protein (mCherry); introduces T to G (A to C) nucleotide change. The premature stop codon is edited into the translatable amino acid - glycine, restoring the wild-type mCherry sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Prime editing is an innovative technology for genome editing that enables the installation of the wide spectrum of gene modifications such as 12 possible base-to-base conversions, small insertions, and deletions, without requiring double-stranded breaks or donor DNA templates. This technology provides high versatility and target specificity, offering the potential to revolutionize medicine by providing novel tools for treating genetic diseases.
+Prime editing relies on specialized prime editors, which usually consist of reverse transcriptase enzyme fused to nickase Cas9, and prime editing guide RNA containing a spacer that specifies the target site. It also includes a scaffold and 3’ extension containing a primer binding site (PBS) and an RT template encoding the desired edit. To initiate prime editing, PE creates a single-strand break in the DNA at the target site to allow reverse transcriptase to access the DNA and synthesize a new DNA strand using pegRNA as a template. Then the information from the edited strand is copied to the complementary strand through the cell’s natural repair pathways.
+mCherry is a red fluorescent protein derived from Discosoma sp. mCherry has an excitation wavelength peak of 587nm and emission wavelength peak at 610nm.
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