Difference between revisions of "Part:BBa K4960030"
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<partinfo>BBa_K4960030 short</partinfo>
 
<partinfo>BBa_K4960030 short</partinfo>
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A structural domain that can specifically recognize the EGFR receptor.
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A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.
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===Profile===
 
===Profile===
 
Name: pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD<br>
 
Name: pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD<br>
 
Base Pairs: 1824bp<br>
 
Base Pairs: 1824bp<br>
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Origin: <br>
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Origin: Synthetic<br>
 
Properties: A structural domain that can specifically recognize the EGFR receptor.<br>
 
Properties: A structural domain that can specifically recognize the EGFR receptor.<br>
  
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===Usage and Biology===
 
===Usage and Biology===
 
Mutant Pvc13-E01-DARPin fused with the DARPin E01 structural domain can enhance the binding ability of PVCs to cells, ultimately enabling targeted delivery of target proteins.[1]
 
Mutant Pvc13-E01-DARPin fused with the DARPin E01 structural domain can enhance the binding ability of PVCs to cells, ultimately enabling targeted delivery of target proteins.[1]
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<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/1.png" alt="" width="800" height="1000" /></p>
 
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Figure 1.Schematic diagram of the influence of different Linkers on the structure of tail fibers
 
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<figure class="figure">
 
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<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/4.png" alt="" width="800" height="1000" /></p>
 
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Figure 2.PVC illustration   
 
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<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/2.png" alt="" width="800" height="600" /></p>
 
  
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Figure 3.Horizontal cutaway view of the fiber region of the syringe.
 
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===Special Design===
 
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Use the flexible linker on both sides of the CKGGRAKDC to affect the structure and function of the tail protein.
 
 
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===Sequence and Features===
 
===Sequence and Features===

Revision as of 14:27, 11 October 2023

pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD A structural domain that can specifically recognize the EGFR receptor.

Profile

Name: pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD
Base Pairs: 1824bp
Origin: Synthetic
Properties: A structural domain that can specifically recognize the EGFR receptor.

Usage and Biology

Mutant Pvc13-E01-DARPin fused with the DARPin E01 structural domain can enhance the binding ability of PVCs to cells, ultimately enabling targeted delivery of target proteins.[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 309
    Illegal BglII site found at 1674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.