Difference between revisions of "Part:BBa K4724002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We needed to purify the | + | We needed to purify the <i>Is</i>PETase, so we attached the 6xHis tag after <i>Is</i>PETase. |
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===Source=== | ===Source=== | ||
Latest revision as of 14:25, 11 October 2023
IsPETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We needed to purify the IsPETase, so we attached the 6xHis tag after IsPETase.
Source
It's from Ideonella sakaiensis.