Difference between revisions of "Part:BBa K4724002:Design"

 
(→‎Source)
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===Source===
 
===Source===
  
−
It's from Ideonella sakaiensis.
+
It's from <i>Ideonella sakaiensis</i>.
  
 
===References===
 
===References===

Revision as of 14:24, 11 October 2023


IsPETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.



Source

It's from Ideonella sakaiensis.

References